Cryopreservation of Zebrafish Ovarian Follicles Essay

Cryopreservation of Zebrafish Ovarian Follicles - Essay ExampleThe technologies in cryopreservation suck in undergone tremendous advances over the last decade. In addition, cryopreservation of oocytes and embryos is also being apply as an effective content of treating infertility. In this technology, the clinical application seeks to ensure the optimal survival of embryos and oocytes that are subsequently thawed and stored for channelise . The aim of this practical experimentation is to compare the slow-cooling procedures with vitrification to analyze and evaluate the most effective and safest procedure as well as to endorse suitable recommendation for the adoption of best practices.To do this, it is necessary to establish and calculate the viability for control, slow-cooling and vitrification samples. Determining the number of cells in the culture is also important for standardising culture conditions and performing straight quantitation experiments . The use of viability test with hemacytometer and typan blue staining will enable us to determine the cell number, the nicety of which is inevitable for accurate test results. Live cells appear colourless and bright under phase contrast, turn the dead cells sustain blue stains and are non-refractive. To facilitate accuracy and consistency of cell counts, we have used a viability counting system. This involves counting viable, live and dead cells in one or more expectant corner squares and recording the cell counts. In order to obtain an accurate cell count, 40 to 70 cells will be counted during the test phase. Therefore, it may be necessary to count more than one extensive corner square.The controlled technique, which is the conservative method used for the purpose of cryopreservation of cells and tissues, is based on the slow-cooling approach. It needs to be appreciated that a large number of non-sensitive cells can be preserved in liquid nitrogen with lower-ranking damage through slow-cooling